Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.
Identifieur interne : 001B38 ( Main/Exploration ); précédent : 001B37; suivant : 001B39Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.
Auteurs : Adam S. Cockrell [États-Unis] ; Kayla M. Peck ; Boyd L. Yount ; Sudhakar S. Agnihothram ; Trevor Scobey ; Nicole R. Curnes ; Ralph S. Baric ; Mark T. HeiseSource :
- Journal of virology [ 1098-5514 ] ; 2014.
Descripteurs français
- KwdFr :
- Analyse de mutations d'ADN, Animaux, Attachement viral, Conformation des protéines, Coronavirus (physiologie), Dipeptidyl peptidase 4 (métabolisme), Données de séquences moléculaires, Humains, Infections à coronavirus (virologie), Modèles moléculaires, Récepteurs viraux (métabolisme), Souris, Spécificité d'espèce, Spécificité d'hôte, Séquence d'acides aminés.
- MESH :
- métabolisme : Dipeptidyl peptidase 4, Récepteurs viraux.
- physiologie : Coronavirus.
- virologie : Infections à coronavirus.
- Analyse de mutations d'ADN, Animaux, Attachement viral, Conformation des protéines, Données de séquences moléculaires, Humains, Modèles moléculaires, Souris, Spécificité d'espèce, Spécificité d'hôte, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Coronavirus (physiology), Coronavirus Infections (virology), DNA Mutational Analysis, Dipeptidyl Peptidase 4 (metabolism), Host Specificity, Humans, Mice, Models, Molecular, Molecular Sequence Data, Protein Conformation, Receptors, Virus (metabolism), Species Specificity, Virus Attachment.
- MESH :
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- physiology : Coronavirus.
- virology : Coronavirus Infections.
- Amino Acid Sequence, Animals, DNA Mutational Analysis, Host Specificity, Humans, Mice, Models, Molecular, Molecular Sequence Data, Protein Conformation, Species Specificity, Virus Attachment.
Abstract
Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics.
DOI: 10.1128/JVI.03764-13
PubMed: 24574399
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A42
- to stream PubMed, to step Curation: 001A42
- to stream PubMed, to step Checkpoint: 001794
- to stream Ncbi, to step Merge: 000D02
- to stream Ncbi, to step Curation: 000D02
- to stream Ncbi, to step Checkpoint: 000D02
- to stream Main, to step Merge: 001B47
- to stream Main, to step Curation: 001B38
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.</title><author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Peck, Kayla M" sort="Peck, Kayla M" uniqKey="Peck K" first="Kayla M" last="Peck">Kayla M. Peck</name></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S" last="Agnihothram">Sudhakar S. Agnihothram</name></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name></author><author><name sortKey="Curnes, Nicole R" sort="Curnes, Nicole R" uniqKey="Curnes N" first="Nicole R" last="Curnes">Nicole R. Curnes</name></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name></author><author><name sortKey="Heise, Mark T" sort="Heise, Mark T" uniqKey="Heise M" first="Mark T" last="Heise">Mark T. Heise</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24574399</idno><idno type="pmid">24574399</idno><idno type="doi">10.1128/JVI.03764-13</idno><idno type="wicri:Area/PubMed/Corpus">001A42</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A42</idno><idno type="wicri:Area/PubMed/Curation">001A42</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A42</idno><idno type="wicri:Area/PubMed/Checkpoint">001794</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001794</idno><idno type="wicri:Area/Ncbi/Merge">000D02</idno><idno type="wicri:Area/Ncbi/Curation">000D02</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D02</idno><idno type="wicri:Area/Main/Merge">001B47</idno><idno type="wicri:Area/Main/Curation">001B38</idno><idno type="wicri:Area/Main/Exploration">001B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.</title><author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Peck, Kayla M" sort="Peck, Kayla M" uniqKey="Peck K" first="Kayla M" last="Peck">Kayla M. Peck</name></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S" last="Agnihothram">Sudhakar S. Agnihothram</name></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name></author><author><name sortKey="Curnes, Nicole R" sort="Curnes, Nicole R" uniqKey="Curnes N" first="Nicole R" last="Curnes">Nicole R. Curnes</name></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name></author><author><name sortKey="Heise, Mark T" sort="Heise, Mark T" uniqKey="Heise M" first="Mark T" last="Heise">Mark T. Heise</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Coronavirus (physiology)</term><term>Coronavirus Infections (virology)</term><term>DNA Mutational Analysis</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Host Specificity</term><term>Humans</term><term>Mice</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Conformation</term><term>Receptors, Virus (metabolism)</term><term>Species Specificity</term><term>Virus Attachment</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Attachement viral</term><term>Conformation des protéines</term><term>Coronavirus (physiologie)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Modèles moléculaires</term><term>Récepteurs viraux (métabolisme)</term><term>Souris</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dipeptidyl peptidase 4</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>DNA Mutational Analysis</term><term>Host Specificity</term><term>Humans</term><term>Mice</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Conformation</term><term>Species Specificity</term><term>Virus Attachment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Attachement viral</term><term>Conformation des protéines</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Modèles moléculaires</term><term>Souris</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><noCountry><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S" last="Agnihothram">Sudhakar S. Agnihothram</name><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><name sortKey="Curnes, Nicole R" sort="Curnes, Nicole R" uniqKey="Curnes N" first="Nicole R" last="Curnes">Nicole R. Curnes</name><name sortKey="Heise, Mark T" sort="Heise, Mark T" uniqKey="Heise M" first="Mark T" last="Heise">Mark T. Heise</name><name sortKey="Peck, Kayla M" sort="Peck, Kayla M" uniqKey="Peck K" first="Kayla M" last="Peck">Kayla M. Peck</name><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B38 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001B38 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24574399
|texte= Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24574399" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |